Figure 6.

No positive influence of 1400W treatment on amacrine cells. A, Representative pictures of amacrine cell population in retinal explants. Amacrine cells were labelled with antibodies against calretinin (green). Nuclei were stained with DAPI (blue). B, On day 4, no significant differences could be observed between the groups. On day 8, a significant loss of amacrine cells in retinae of the CoCl₂ group was detected. The iNOS inhibitor treatment could not protect the amacrine cells. C, No differences in the relative expression of PVALB mRNA were observed on day 4. On day 8, the relative expression of PVALB was reduced in all groups compared with the control and significantly in comparison with the iNOS‐inh. treatment. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer. Scale bar = 20 µm. All data are shown as mean ± SEM. *P < .05; **P < .01; ***P < .001