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. 2020 Mar 4;24(7):4312–4323. doi: 10.1111/jcmm.15091

Table 2.

qRT‐PCR primer pairs in 5′‐3′ directions. The listed primer pairs were used in qRT‐PCR experiments, while β‐actin and RLP4 were used as housekeeping genes

Gene Oligonucleotide sequence
HIF‐1α for TTACAGCAGCCAGATGATCG
HIF‐1α rev TGGTCAGCTGTGGTAATCCA
CD11b for AGAAGGAGACACCCAGAGCA
CD11b rev GTAGGACAATGGGCGTCACT
PVALB for CAACGCTGAGGACATCAAGA
PVALB rev TGACAGGTCTCTGGCATCTG
β‐Actin for CTCTTCCAGCCTTCCTTC
β‐Actin rev GGGCAGTGATCTCTTTCT
RPL4 for CAAGAGTAACTACAACCTTC
RPL4 rev GAACTCTACGATGAATCTTC
HSP70 for ATGTCCGCTGCAAGAGAAGT
HSP70 rev GGCGTCAAACACGGTATTCT
iNOS for TGTTCAGCTGTGCCTTCAAC
iNOS2 rev CAGAACTGGGGGTACATGCT
CCL2 for TCTCCAGTCACTGCTA
CCL2 rev TCCAGGTGGCGGAGTC

Abbreviations: for, forward; rev, reverse.