Figure 4.

Functional annotation and network analyses for green module. (A) GO enrichment analysis results of green module are shown. The GO terms are divided into two groups: BP and CC. (B) Network depiction of green module. Nodes correspond to genes, and lines correspond to connections, with the top 250 connections in the green module shown. (C) GSEA results show that several gene sets enriched in GBMs with high expression levels of SRPX2. (D) mRNA expression levels of PLOD3 and SLC20A1 among molecular subtypes and glioma grades in different data sets. (E) IHC staining shows the protein expression levels and cellular localizations of PLOD3 and SLC20A1 in normal brain tissues, LGGs and HGGs. (F) Survival analyses between high 50% and low 50% PLOD3 expression group of all gliomas or GBMs in Rembrandt data set. Means ± SEM; one‐way ANOVA with Tukey's post hoc test; ns, P > .05, *, P < .05; **, P < .01, ***, P < .001, ****, P < .0001. IHC, immunohistochemistry; LGGs, low‐grade gliomas; HGGs, high‐grade gliomas