Figure 1.
Effects of hydrogen gas and two reagents on normal PC12 cells. Prepared PC12 cells that were cultured on 6‐well plates were randomly divided into control group, control + hydrogen intervention group (Con + H2 group), control + miR‐21 inhibition group (Con + M group) and control + PI3K inhibition group (Con + P group). Each group included 10 wells of PC12 cells. The cells in the Con + H2 group were cultured in hydrogen‐rich DMEM for 48 h. MiR‐21 antagomir was transfected into PC12 cells in the Con + M group. The cells in the Con + P group were treated with PI3K blocker LY294002 for 30 min. After these interventions, cell viability was evaluated using a commercial MTT proliferation assay kit. Neuronal injury was evaluated by measurement of lactate dehydrogenase (LDH) concentration using a commercial LDH detection kit. Percentage of TUNEL+ cells was obtained using a commercial TUNEL kit. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in PC12 cells were measured using their commercial test kits. The results were expressed as mean ± standard deviation. Differences in these markers among the four groups were compared using one‐way ANOVA with LSD t test. ‘†’ indicates P > .05