Skip to main content
. 2020 Feb 27;24(7):4223–4232. doi: 10.1111/jcmm.15082

Figure 2.

Figure 2

SEZ6L2 promotes CRC cell growth in vitro. A, Western blotting analysis of SEZ6L2 expression in human intestinal epithelial cells (HIEC) and CRC cells lines. GAPDH was used as a loading control. B, Western blotting analysis of SEZ6L2 expression in HCT116 and HT29 cells that were stably infected with lenti‐shSEZ6L2‐1 and lenti‐shSEZ6L2‐2. GAPDH was used as a loading control. C, Detection of cell viability at 0, 24, 48 and 72 h after cell plating by CCK‐8 assay. The absorbance of OD450 nm was recorded (n = 4, **P < .01). D, Colony formation analysis of HCT116 and HT29 cells that were stably infected with lenti‐shSEZ6L2‐1 and lenti‐shSEZ6L2‐2 (14 d after cell plating). The number of colonies in each well was counted and analysed (n = 4, **P < .01). E, Invasion analysis of HCT116 and HT29 cells that were stably infected with lenti‐shSEZ6L2‐1 and lenti‐shSEZ6L2‐2. The number of invaded cells in each well was counted and analysed (n = 4, ns, no significant difference)