Figure 4.

Knockdown of SEZ6L2 promotes caspase‐dependent apoptosis in CRC. A and B, Detection of cell viability at 72 h after cell plating by CCK‐8 assay. The relative cell viability was analysed. (n = 4, **P < .01, compared with shNC‐DMSO group; ns, no significant difference, compared with shNC‐z‐VAD‐FMK group). C and D, Colony formation analysis of HCT116 cells that were treated with DMSO and z‐VAD‐FMK (14 d after cells plating). The number of colonies in each well was counted and analysed (n = 4, **P < .01; compared with shNC‐DMSO group; ns, no significant difference, compared with shNC‐z‐VAD‐FMK group)