Figure 2.

DBA/2J ‘Pure ocular hypertensive’ mice display IOP elevation without pigment dispersion and angle closure. A‐C, Representative images for the detection of pigment dispersion in mice using a double goniolens microscope (Phoenix Research Laboratory). A′‐C′, Representative microscopic images of mouse anterior eye. D, D′, Representative optical coherence tomography (OCT) image of mouse anterior segment angle. Arrows in B, C indicate pigment dispersion. Ages as indicated. E, Representative Fontana‐Masson (FM) stained image of the TM region. E′ is the unstained control. Images were selected from n = 10 eyes from n = 10. A modified protocol was used for solution‐phase quantification. F, Quantification of pigment (using modified solution‐phase FM staining) and IOP in DBA/2J mice (7.5‐8.5 mo of age). A total of 104 mice eyes were utilized and each eye was subjected to three measurements. Each data point represents mean of three independent measurements. There is no correlation between pigment dispersion and IOP elevation. The cohort of hypertensive mouse utilized has been indicated using a hollow circle (arrow). G, A representative OCT image of an 8.5‐month‐old mouse with IOP 26 mm of Hg. H, DBA/2J mice (7.5‐8.5 months old, n = 24 animal eyes) with normal and elevated IOP showing low levels of pigment estimated using spectrophotometric FM method at end‐point. Each eye was subjected to three independent readings