Figure 2.

Transcriptome heat map of Huh‐7‐trans and Huh‐7‐DN populations. Two aliquots of Huh‐7, Huh‐7‐trans and Huh‐7‐DN cells were used for RNA extraction in each cell type. B, Heat map of representative differential expression of genes in drug resistance, hedgehog signalling, stemness, cancer progression and hepatocyte‐specific genes from RNA‐sequencing data. A, Venn diagrams of significant differentially expressed genes between Huh‐7‐trans and Huh‐7‐DN vs Huh‐7 cells. FDR < 0.01 & log2|FC| ≥ 2 was considered as significantly differential gene expression. Representative of differential gene expression levels of hepatocyte‐specific genes (C‐F), EMT‐associated transcription factors (G‐J) and cancer progressive genes (K‐N) was verified by quantitative RT‐PCR using Huh‐7 cells as controls (n = 3). *P < .05; **P < .01 compared with controls