Figure 4.

Effects of CMTM5-v1 on the efficacy of Gefitinib. (a) Transfected PC3 cells were plated in 96-well plates until adherent and then incubated in normal growth medium supplemented with 10 μM Gefitinib or drug vehicle (0.1% DMSO). Twenty-four hours after treatment, the MTT assay was used to detect cell viability. Data represent the percentages related to vector-transfected cells treated with vehicle. All values were means ± SEM from three independent experiments. (b) Transfected PC3 cells were incubated in normal growth medium with 10 μM Gefitinib or drug vehicle (0.1% DMSO) for 24 h. Whole-cell lysates were immunoblotted with the indicated antibodies.