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. 2020 Mar 31;11(13):3701–3712. doi: 10.7150/jca.36372

Figure 5.

Figure 5

Anticancer activity of MET + JS-K on RCC cells was reversed by NAC and exacerbated by GSSG. The ACHN and A498 cells were cultured with 100 μM NAC or 5 μM GSSG for 24 h and then treated with or without MET (5 mM) + JS-K (1 µM). (A) Cells were visualized by microscopy (100×). (B) Cell survival was detected by MTT assay. (C) Cytotoxicity was investigated using an LDH Cytotoxicity Assay Kit. The data are presented as the mean ± SD for three experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 compared with untreated cells.