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. 2020 Apr 10;2020:6981520. doi: 10.1155/2020/6981520

Figure 7.

Figure 7

After (a) MGC803, (b) SGC7901, and (c) MKN74 cells were treated with β-asarone (60 μg·ml−1) and CoCl2 (200 μM) single or combined for 24 h the mRNA levels of tumor glycolysis related genes (HIF-1α, PDK1, PDK4, and c-myc) were determined by RT-PCR. β-Actin was taken as a loading control. (∗P < 0.05, compared with the control group; ΔP < 0.05, compared with CoCl2 group). (d) MGC803, (e) SGC7901, (f) MKN74. Western blotting analysis of tumor glycolysis related proteins after GC cells were treated with β-asarone (60 μg·ml−1), CoCl2 (200 μM), and H2O2 (100 μM) single or combined for 24 h (∗P < 0.05, ∗∗P < 0.01, compared with control group; #P < 0.05, ##P < 0.01, compared with H2O2 group; ΔP < 0.05, ΔΔP < 0.01, compared with CoCl2 group).