Table 2.
qRT-PCR results of comparison between uninduced control and OS-induced AVICs.
| OS-induced AVICs | |||
|---|---|---|---|
| Osteogenic marker genes | Osteopontin, Runx2, ALP, Msx2, Osterix | All are upregulated | |
|
| |||
| Negative regulatory molecule of bone cell differentiation | Asporin | Downregulated | |
|
| |||
| AVICs marker genes | Fibroblast | Col1a1 | Downregulated |
| Fibroblast | Vimentin | Downregulated | |
| Myofibroblast | Sm22α | Upregulated | |
| Extracellular matrix molecule | Fibronectin | Upregulated | |
| Cell adhesion molecule | N-Cadherin | Upregulated | |
| Matrix metalloproteinase | Adamts5 | Upregulated | |
| Matrix metalloproteinase | Adamts9 | Downregulated | |
|
| |||
| Intracellular phosphate level regulatory gene | Na-dependent Pi transporter | PiT2 | Upregulated |
|
| |||
| Signaling-specific markers genes | Wnt signaling | β-Catenin | Upregulated |
| Wnt3a | Upregulated | ||
| Dkk1 | Downregulated | ||
|
| |||
| Other signaling-specific marker genes | Bmp2 | Upregulated | |
| Smad 1 | Downregulated | ||
| Smad 5 | Upregulated | ||
| Smad 8 | Upregulated | ||
| Notch 1 | Upregulated | ||
| Sox 9 | Downregulated | ||