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qRT-PCR results of comparison among OS-induced control, OS+Xav939-, and OS+Xav939+rhAsp-treated AVICs.
| OS+Xav939-conditioned AVICs | OS+Xav939+rhAsp-conditioned AVICs | ||
|---|---|---|---|
| Negative regulatory molecule of bone cell differentiation | Asporin | Unchanged | Upregulated |
| Osteogenic marker genes | Osteopontin, Runx2 | Unchanged | Downregulated |
| Wnt signaling-specific markers genes | β-Catenin | Downregulated | Downregulated |
| Wnt3a | Downregulated | Downregulated | |
| Dkk1 | Upregulated | Upregulated | |
