Table 1.
Identification of protein spots
| Protein spot No. | Score SEQUEST | Queries matched | Sequence coverage (%) | pI (Expa)/MW (Exp) (kDa) | Identified proteinb (UniProtKB/Swiss-Prot ID) |
|---|---|---|---|---|---|
| 1 | 98.53 | 10 | 51 | 6.81(4.05)/26.85(24) | Triosephosphate isomerase (P48500) |
| 2 | 155.96 | 13 | 56 | 6.73(4.46)/28.83 (26) | Phosphoglycerate mutase (P25113) |
| 3 | 67.64 | 8 | 51 | 7.37(5.60)/25.55 (27) | Dihydropteridine reductase-2 (P11348) |
| 4 | 186.56 | 14 | 31 | 5.25(5.76)/55.49(37) | Pyridine nucleotide-disulfide oxidoreductase (Q68FT6) |
| 5 | 98.45 | 12 | 46 | 6.47(5.91)/39.14 (33) | Phosphotriesterase-related protein (Q63530) |
| 6 | 595.06 | 29 | 76 | 6.11(6.00)/45.80(45) | Aminoacylase-1A (Q6AYS7) |
| 7 | 68.57 | 8 | 38 | 5.81(6.90)/31.42(27) | N(G),N(G)-dimethylarginine dimethylaminohydrolase (O08557) |
| 8 | 184.72 | 17 | 52 | 7.56(7.40)/44.54(40) | Phosphoglycerate kinase 1 (P16617) |
a) Exp, experimental
b) List of peptides in Additional file 1