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. 2020 Apr 21;13:174. doi: 10.1186/s13071-020-04057-7

Table 1.

PCR primers used for detection of selected vector-borne pathogens: Ehrlichia spp., Anaplasma spp., Hepatozoon canis, Babesia spp., Theileria spp. and Leishmania spp. in dogs from Kathmandu, Nepal

Species Target gene Forward primer (5’-3’) Reverse primer (5’- 3’) References
Ehrlichia spp.; Anaplasma spp. 16S rRNA GCAAGCYTAACACATGCAAGTCG GGATTATACAGTATTACCCAYCATTTCTARTG [6]
Hepatozoon canis 18S rRNA CTTACCGTGGCAGTGACGGT ATTGTTATTTCTTGTTACTACCTCTCTCAAAC
Piroplasmida (Babesia/Theileria spp.) GACGATCAGATACCGTCGTAGTCC CAGAACCCAAAGACTTTGATTTCTCTC
Leishmania spp. Kinetoplast minicircle DNA AACTTTTCTGGTCCTCCGGGTAG ACCCCCAGTTTCCCGCC [7]

Abbreviation: rRNA ribosomal ribonucleic acid