. 2020 Apr 21;25:14. doi: 10.1186/s40001-020-00412-7

Table 2.

Detailed clinical, demographic and molecular characteristics of 42 M. tuberculosis isolates in 16 (Cluster I to Cluster XVI) clusters

Cluster no.	Clinica specimen	Isolate no.	Year of isolation	Patient’s nationality	Spoligotyping data		Genetic alteration detected in
Cluster no.	Clinica specimen	Isolate no.	Year of isolation	Patient’s nationality	SIT	Mtb family	rpoB	katG	inhA	pncA	embB	rpsL	rrs	gidB	rpsA
I	Sputum	KM06-153	2006	Indian	255	Beijing	TCG456TTG	ACG315ACC	WT	WT	ATG306GTG	AAG43AGG	WT	N. D.	N. D.
	CSF	KM09-22	2009	Indian	255	Beijing	TCG456TTG	ACG315ACC	WT	WT	ATG306GTG	AAG43AGG	WT	N. D.	N. D.
	Sputum	KM13-37	2013	Indian	1	Beijing	TCG456TTG	ACG315ACC	WT	WT	ATG306GTG	AAG43AGG	WT	N. D.	N. D.
	FNA	KM16-06	2016	Nepalese	1	Beijing	TCG456TTG	ACG315ACC	WT	WT	ATG306GTG	AAG43AGG	WT	N. D.	N. D.
	FNA	KM17-03	2017	Indian	1	Beijing	TCG456TTG	ACG315ACC	WT	WT	ATG306GTG	AAG43AGG	WT	N. D.	N. D.
II	Sputum	KM14-58	2014	Nepalese	1	Beijing	TCG456TTG	ACG315ACC	WT	GTG139GCG	ATG306GTG	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
II	Sputum	KM14-69	2014	Indian	1	Beijing	TCG456TTG	ACG315ACC	WT	GTG139GCG	ATG306GTG	AAG43AGG	WT	GCA205GCG	WT
III	Sputum	KM08-501	2008	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	GGT139GTT	ATG306GTG	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Sputum	KM08-502	2008	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	GGT139GTT	ATG306GTG	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Sputum	KM09-207	2009	Indian	1	Beijing	TCG456TTG	ACG315ACC	WT	GGT139GTT	ATG306GTG	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
IV	Sputum	KM12-05	2012	Ethiopian	21	CAS1-Kili	TCG456TTG	ACG315ACC	WT	Ins193A (FS) + TCC65TCT	ATG306GTG	AAG88AGG	WT	N. D.	N. D.
	Sputum	KM12-17	2012	Ethiopian	1144	T1	TCG456TTG	ACG315ACC	WT	Ins193A (FS) + TCC65TCT	ATG306GTG	AAG88AGG	WT	N. D.	N. D.
	Sputum	KM15-08	2015	Ethiopian	21	CAS1-Kili	TCG456TTG	ACG315ACC	WT	Ins193A (FS) + TCC65TCT	ATG306GTG	AAG88AGG	WT	N. D.	N. D.
V	Sputum	KM07-333	2007	Indonesian	Orphan	N. A.	TCG456TTG	ACG315ACC	WT	WT	WT	WT	WT	N. D.	N. D.
V	Sputum	KM10-23	2010	Indian	355	EAI3-IND	TCG456TTG	ACG315ACC	WT	WT	WT	WT	WT	N. D.	N. D.
VI	Sputum	KM07-293	2007	Filipino	194	LAM2	TCG456TTG	ACG315ACC	WT	WT	CAG497CGG	WT	WT	N. D.	N. D.
VI	Sputum	KM12-01	2012	Filipino	25	CAS1-Delhi	TCG456TTG	ACG315ACC	WT	WT	CAG497CGG	WT	WT	N. D.	N. D.
VII	Sputum	KM09-202	2009	Ethiopian	47	H1	GTC176TTC	ACG315ACC	WT	WT	WT	WT	WT	N. D.	N. D.
VII	Sputum	KM15-17	2015	Indian	47	H1	GTC176TTC	ACG315ACC	WT	WT	WT	WT	WT	N. D.	N. D.
VIII	Sputum	KM14-67	2014	Ethiopian	149	T3-ETH	TCG456TTG	ACG315ACC	WT	− 11 A/G	ATG306ATC	WT	WT	GGT69GAT	WT
VIII	Sputum	KM15-21	2015	Ethiopian	149	T3-ETH	TCG456TTG	ACG315ACC	WT	− 11 A/G	ATG306ATC	WT	WT	GGT69GAT	WT
IX	Sputum	KM07-283	2007	Indian	26	CAS1-Delhi	TCG456TTG	ACG315ACC	WT	TCC65TCT	ATG306ATA	WT	WT	N. D.	N. D.
	Sputum	KM14-68	2014	Indian	Orphan	N. A.	TCG456TTG	ACG315ACC	WT	TCC65TCT	ATG306ATA	WT	WT	N. D.	N. D.
	Sputum	KM17-20	2017	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	CAG497CGG	AAG43AGG	WT	GAA92GAC + GCA205GCG	WT
X	Sputum	KM17-22	2017	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	CAG497CGG	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Sputum	KM17-73	2017	Indian	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	CAG497CGG	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Pus	KM11-503	2011	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	GGC406GAC	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Sputum	KM14-56	2014	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	GGC406GAC	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
XI	Sputum	KM15-13	2015	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	GGC406GAC	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Sputum	KM15-26	2015	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	GGC406GAC	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Sputum	KM17-02	2015	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	GGC406GAC	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
	Sputum	KM17-69	2017	Kuwaiti	1	Beijing	TCG456TTG	ACG315ACC	WT	− 11 A/G	GGC406GAC	AAG43AGG	WT	GAA92GAC + GCA205GCG	CGA212CGC
XII	Sputum	KM16-32	2016	Egyptian	19	EAI2-Manila	CAC451TAC	ACG315ACC	− 15 C/T	GAA37AAA	CTG355CTA + GAG378GCG	WT	WT	GTG110GTT + GCA205GCG	WT
XII	Sputum	KM17-01	2017	Filipino	19	EAI2-Manila	CAC451TAC	ACG315ACC	− 15 C/T	GAA37AAA	CTG355CTA + GAG378GCG	WT	WT	CTC59TTC + GTG110GTT + GCA205GCG	WT
XIII	Pus	KM07-297	2007	Indian	Orphan	N. A.	CAC451GAC	WT	− 15 C/T	TCC65TCG + Ins 453T (FS)	ATG306CTG	WT	WT	N. D.	N. D.
XIII	FNA	KM11-502	2015	Indian	3361	T1	CAC451GAC	WT	− 15 C/T	TCC65TCG + Ins 453T (FS)	ATG306CTG	WT	WT	N. D.	N. D.
XIV	Sputum	KM06-48	2006	Egyptian	53	T1	TCG456TTG	WT	− 15 C/T	WT	WT	WT	WT	N. D.	N. D.
XIV	Tissue	KM06-277	2006	Filipino	19	EAI2-Manila	TCG456TTG	WT	− 15 C/T	WT	WT	WT	WT	N. D.	N. D.
XV	Sputum	KM16-33	2016	Indian	8	EAI3/EAI5	CAC451TAC	ACG315ACC	WT	CTG35CCG	ATG306GTG + GAG378GCG	AAG43AGG	WT	GTG110GTT + GCA205GCG	WT
XV	Sputum	KM17-06	2017	Filipino	8	EAI3/EAI5	CAC451TAC	ACG315ACC	WT	CTG35CCG	ATG306GTG + GAG378GCG	AAG43AGG	WT	GTG110GTT + GCA205GCG	WT
XVI	Sputum	KM07-231	2007	Indian	Orphan^a	CAS1-Delhi	ATG440ATA + GAC441TAC	ACG315ACC	WT	TCC65TCT	GGC406TGC	WT	WT	GCA205GCG + Del 350G (FS)	WT
XVI	Sputum	KM07-252	2007	Syrian	Orphan^a	CAS1-Delhi	ATG440ATA + GAC441TAC	ACG315ACC	WT	TCC65TCT	GGC406TGC	WT	WT	GCA205GCG + Del 350G (FS)	WT

Clusters containing MDR-TB strains with identical patterns and isolated within a period of nearly 2 years are shown as underlined. Synonymous mutations are italicized

N. A., not applicable; N. D., not done; CSF, cerebrospinal fluid; FNA, fine needle aspirate; SIT, shared international type; Mtb family, M. tuberculosis family; WT, wild-type sequence; Ins, insertion mutation; (FS), frame shift mutation, fine needle aspirate

^aBoth isolates displayed identical spoligotyping pattern