Table 2.

PCR mixture used in this study

PCR	Bp (flaB)		Bbss (ospA)
	Working concentration	Reaction volume (µl)	Working concentration	Reaction volume (µl)
Master mixa	1×	10	1×	10
Forward primer	600 nM	1.2	900 nM	0.64
Reverse primer	600 nM	1.2	900 nM	0.64
Probe	200 nM	0.8	100 nM	0.97
Reference dyeb		0.1	–	–
Nuclease-free water		4.2		5.25
Template DNA		2.5		2.5

^aQuantiNova probe PCR master mix (Qiagen, Hilden, Germany)

^bQN ROX reference dye (Qiagen)

Abbreviations: Bbss, Borrelia burgdorferi sensu stricto; Bp, Borrelia persica