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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: J Invest Dermatol. 2019 Mar 13;139(9):2029–2038.e3. doi: 10.1016/j.jid.2019.02.025

Figure 3. Cox-2 knockdown in fibroblasts decreases PGE2 secretion and promotes TGF-β-mediated induction of α-SMA expression.

Figure 3.

(a-c) NHDFs were infected with lentivirus expressing control non-targeting shRNA (NT), Cox-2 targeting shRNAs or uninfected (UI) and recovered for 48 hrs. NHDFs were serum starved overnight followed by 2 ng/mL of TGF-β for 3 days; (a) α-SMA expression was measured by western blot (normalized to GAPDH). n=3, 1-way ANOVA, *p<0.05; error bars are +/− SEM; (b) secreted PGE2 concentration was measured by ELISA; n=3, 1-way ANOVA, *p<0.05; error bars are +/− SEM. (c) NHDFs incubated with TGF-β with or without 500 nM PGE2 for 3 days, and α-SMA expression was measured by western blot (normalized to GAPDH). n=3, 2-way ANOVA analysis, * p<0.05; error bars are +/− SEM.