Figure 1.

Physiologic and pharmacologic ketosis is not temporally associated with peripheral glucagon levels. Mice were fasted overnight and refed at 8:00 a.m. for 30 min, and glycemia (A), βOHB (B), NEFA (C), insulin (D), and glucagon (E) were measured. Mice were fasted for 3 h and then administered PBS or dapagliflozin (10 mg/kg), and glycemia (F), βOHB (G), NEFA (H), insulin (I), and glucagon (J) were measured over the following 6 h. Data are shown as mean ± SEM. *P < 0.05. Data were analyzed by a two-tailed Student t test (A–E) or two-way ANOVA (F–J).