1H NMR and HSQC NMR spectra of PPS
a.
1H NMR spectrum with anomeric region and O-methyl -acetyl region expansions
b. Anomeric region of HSQC NMR spectrum
Apex number (e.g. 1Xyl) shows the number of the carbon observed in signal. Xyl – sulfated xylose (all position except specified), MGA – branching 4-O-methyl-2,3-di-O-sulfated glucuronic acid, Ac – acetyl group, Me – methyl group, Py – pyridine moiety, ΔXyl – C2-C3 double bond, * different from MGA-Xyl due to sequence effects. Xyl*(n) xylose residues related to sequence effects not fully elucidated.