Figure 2.

Solution-phase stability analysis of TTR variants using a VT-ESI source. (A) Relative abundance of tetrameric WT-TTR to total ions as a function of collision voltage (25 °C). Data are fitted with an exponential fit to get I₅₀, which represents the voltage that 50% of tetramers remains intact. (B) I₅₀ values for WT-, CT-, and FT₂-TTR at 25, 42, 62, and 78 °C without (solid) and with (dashed) T₄ in a 1:2 molar ratio (TTR/T₄). Measurements are obtained for three different replicates (n = 3) with error bars shown as ± standard deviation.