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Confocal microscopy images of representative MDA-MB-231 3D aggregates within small (a,c) and large (b,d) microcapsules at day 14. Aggregates were GFP-transfected (green) and nuclear stained with DAPI (blue). Cross-sections (a,b), and mean intensity projections (MIP) (c,d) demonstrate the shape of the aggregates, as well as the limitations of confocal imaging for capturing the full 3D geometry of the larger aggregates.
