Table 1.

Primers and probes used in multiplex real-time PCR run in five reaction mixtures, A-E

Mix	Primer/Probe	Sequence	Origin	Reporter
A	PIV-1_F	GTTGTCAATGTCTTAATTCGTATCAATAATT	Watzinger et al. (2004)
A	PIV-1_R	GTAGCCTMCCTTCGGCACCTAA	Watzinger et al. (2004)
A	PI V-l_ Probe	TAGGCCAAAGATTGTTGTCGAGACTATTCCAA	Watzinger et al. (2004)	FAM-TAMRA
A	PIV-2_F	GCATTTCCAATCTTCAGGACTATGA	Watzinger et al. (2004)
A	PIV-2_R	ACCTCCTGGTATAGCAGTGACTGAAC	Watzinger et al. (2004)
A	PIV-2_Probe	CCATTTACCTAAGTGATGGAATCAATCGCAAA	Watzinger et al. (2004)	FAM-TAMRA
A	PIV-3_F	To be published elsewhere	a
A	PIV-3_R	To be published elsewhere	a
A	PIV-3_Probe	To be published elsewhere	a	FAM-BHQ
B	IfB_F	AAATACGGTGGATTAAATAAAAGCAA	a
B	IfB_R	CCAGCAATAGCTCCGAAGAAA	a
B	IfB_Probe	CACCCATATTGGGCAATTTCCTATGGC	a	JOE-TAMRA
B	RSV_F	GCAAATATGGAAACATACGTGAACA	This publ
B	RSV_R	GCACCCATATTGTWAGTGATGCA	This publ
B	RSV_Probe	CTTCACGAAGGCTCCACATACACAGCWG	This publ	FAM-BHQ
B	NL63_F	ACGTACTTCTATTATGAAGCATGATATTAA	Gunson et al. (2005)
B	NL63_R	AGCAGATCTAATGTTATACTTAAAACTACG	Gunson et al. (2005)
B	NL63_P	ATTGCCAAGGCTCCTAAACGTACAGGTGTT	Gunson et al. (2005)	HEX-BHQ1
C	MPV_F	ATGTCTCTTCAAGGGATTCACCT	This publ
C	MPV_R	AMAGYGTTATTTCTTGTTGCAATGATGA	This publ
C	MPV_Probe	CATGCTATATTAAAAGAGTCTCARTAC	This publ	FAM-MGB
C	Ch. pneumoniae_F	CAAGGGCTATAAAGGCGTTGCT	This publ
C	Ch. pneumoniae_R	ATGGTCGCAGACTTTGTTCCA	This publ
C	Ch. pneumoniae_P	TCCCCTTGCCAACAGACGCTGG	This publ	CY5-BHQ
C	EV_F	CATGGTGYGAAGAGTCTATTGAGCTA	This publ
C	EV_R	GGACACCCAAAGTAGTCGGTTC	This publ
C	EV_probe	CGGCCCCTGAATGCGGCTAATC	This publ	HEX-BHQ1
D	IfA_F	AAGACCAATCCTGTCACCTCTGA	Ward et al. (2004)
D	IfA_R	CAAAGCGTCTACGCTGCAGTCC	Ward et al. (2004)
D	IfA_Probe	TTTGTGTTCACGCTCACCGT	Ward et al. (2004)	FAM-MGB
D	AdV_F	GCCACGGTGGGGTTTCTAAACTT	Heim et al. (2003)
D	AdV_R	GCCCCAGTGGTCTTACATGCACATC	Heim et al. (2003)
D	AdV_Probe	TGCACCAGACCCGGGCTCAGGTACTCCGA	Heim et al. (2003)	HEX-BHQ1
D	OC43_F	CGATGAGGCTATTCCGACTAGGT	Gunson et al. (2005)
D	OC43_R	CCTTCCTGAGCCTTCAATATAGTAACC	Gunson et al. (2005)
D	OC43_Probe	TCCGCCTGGCACGGTACTCCCT	Gunson et al. (2005)	CY5-BHQ2
E	RV_F1	GGTGTGAAGAGCCSCRTGTGCT	This publication
E	RV_F2	GGTGTGAAGACTCGCATGTGCT	This publication
E	RV_F3	GGGTGYGAAGAGYCTANTGTGCT	This publication
E	RV_R3	GGACACCCAAAGTAGTYGGTYC	This publication
E	RV_probe	CCGGCCCTGAATGYGGCTAAYC	This publication	FAM-BHQ1
E	Mycoplasma_F	GGAATCCCAATGCACAAGAACA	This publication
E	Mycoplasma_R	GCTTTGGTCAACACATCAACCTT	This publication
E	Mycoplasma_Probe	GCCTTGAAGGCTGGGTTTGCGCTA	This publication	CY5-BHQ
E	229E_F	CAGTCAAATGGGCTGATGCA	Gunson et al. (2005)
E	229E_R	AAAGGGCTATAAAGAGAATAAGGTATTCT	Gunson et al. (2005)
E	229E_P	CCCTGACGACCACGTTGTGGTTCA	Gunson et al. (2005)	HEX-BHQ1

^aPersonal communication from Dr Lars Nielsen, Copenhagen. FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine; JOE, dichloro-2′,7′-dimethoxy-6-carboxyfluorescein; HEX, hexachlorofluorescein; CY5, Cyanine-5; BHQ, black-hole quencher; MGB, minor groove binder.