. 2014 Nov 15;43(1):31–36. doi: 10.1016/j.biologicals.2014.10.004

Table 2.

Conditions for real time PCR.

Virus	Primer	Sequence (5′-3′)	Amplicon size (nt)	Cycling conditions	Reference
Circovirus 1 et 2	Forward	TGGCCCGCAGTATTTTGATT	72	45 cycles 95 °C: 10 s 58 °C: 5 s 72 °C: 10 s	[34]
Circovirus 1 et 2	Reverse	CAGCTGGGACAGCAGTTGAG	72	45 cycles 95 °C: 10 s 58 °C: 5 s 72 °C: 10 s	[34]
Bocavirus	Forward	GTACCGATCTATGATGTATCAC	231	45 cycles 95 °C: 10 s 47 °C: 5 s 72 °C: 10 s	[25]
Bocavirus	Reverse	AAAGGACCCAARTAATTAT	231	45 cycles 95 °C: 10 s 47 °C: 5 s 72 °C: 10 s	[25]
PPV7	Forward	TGGTCGTGATGATGATGGG	104	45 cycles 95 °C: 10 s 56 °C: 5 s 72 °C: 10 s
PPV7	Reverse	CGCAGAGAAAGCCAAACAAG	104	45 cycles 95 °C: 10 s 56 °C: 5 s 72 °C: 10 s