Fig. 2.

Recombination analyses of porcine reproductive and respiratory syndrome virus KU-N1202. (A) Similarity plot analysis of KU-N1202 (query) compared to CP07–626–2 (blue) and VR-2332 (red). Yellow indicates recombination region between 13,351 and 14,705, which covers ORF5, ORF6, and partial ORF7. Phylogenetic trees of major (B) and minor (C) parental regions. The major parental region of KU-N1202 was found to be related to Korean MN184-like strains, but the minor parental region of KU-N1202 was closely related to VR-2332. The beginning (D) and ending (E) of recombination breakpoints were elucidated. The beginning breaking point (black box) was found to be located in the transcription-regulating sequence (gray shade box) at the 5′ end of sub-genomic RNA 5. Left and right dashes indicate the stop codon of ORF5 and the start codon of ORF6, respectively. The ending breakpoint (black box) was found to be located in the middle region of ORF7.