Fig. 5.

Measurement of TNF-α and IFN-γ proteins in serum and amniotic fluid. ELISA was used to measure the amount of immunoreactive cytokine proteins in serum (open circles) and amniotic fluid (closed circles) in infected fetuses and fetuses from mock-infected dams. Each cytokine was measured in 100 μl of sample and samples from control and infected fetuses assayed on a single ELISA plate. Each data point is the mean A450 values of triplicate and duplicate samples for serum and amniotic fluids, respectively. The horizontal lines show the A450 values for the cytokine standards.