Fig. 2.

Spread of AdGFP in cell monolayers and transfer to HEK 293. AdGFP spreads slowly in monolayers of R06E and R05T cells without cytopathic effect. Avian CR cells can be infected but vector does not spread. AdGFP spreads rapidly with fulminant CPE in 293 cells. (B) AdGFP is lost in Rousettus cells within 5 passages. Infected R06E cells at passage 3 were mixed with an equal number of 293 sentinel cells. AdGFP infrequently is transferred from R06E (weak GFP signal) to 293 cells (bright GFP signal without CPE at day 4) by cell–cell contact but not via supernatant. Fulminant CPE in 293 cells at day 8, except for weak GFP expression no effect on Rousettus cells.