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. 2009 Jun 18;145(1):54–62. doi: 10.1016/j.virusres.2009.06.007

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Copyright © 2009 Elsevier B.V. All rights reserved.

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Fig. 4 — Kinetic for MVA replication declines in R06E at high passage. (A) Appearance of MVA cytopathic effect is delayed in high passage R06E (but not in R05T) and cultures of R06E have to sub-passaged until infection is visible. (B) Replication of MVA is rapid in R05T and CR, slower in BHK and clearly delayed in the high-passage R06E cell line. This experiment was performed after approximately 65 weeks of continuous cultivation. Infection was performed with a MOI of 0.1 corresponding to an input virus concentration of 2 × 10⁴ pfu/ml in all cultures at day 0.