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Sensitivity of the quantitative RT-PCR method in detecting: (A) hRSV subgroup A plasmid; (B) hRSV subgroup B plasmid dilutions; and (C): C+, C− and plasmid subgroup A dilutions used for quantification; lanes 1–10: clinical subgroup A (lanes 3, 4, 5, 8, and 9) and B (lanes 1 and 6) strains. (D) Quantification curve. MW, molecular weight markers; N (lanes 2, 7, 10), negative samples; RNA Ic, armored RNA internal control; C+, positive control; C−, negative control.
