Fig. 4.

Identification of the artificially introduced silent point mutations in the BSD1 genome. RT-PCR of total cellular RNA extracted from BSD1-infected MDBK cells was performed using primer pairs which span the genetic markers in the BSD1 genome. The cDNA products were purified and sequenced directly. Only a portion of the sequencing data containing the silent point mutations is presented: (A) T7575C; (B) G7872C; (C) T9022A; (D) A10912G; and (E) A12264G. The primer pairs used for RT-PCR were: BVD6891 and BVD7920 for panel (A) and (B); BVD8905 and BVD9450 for panel (C); BVD9985 and BVD11155 for panel (D); and BVD12043 and SD1-SdaI-PacI for (E). The silent point mutations are indicated with white nucleotide letters on black in each panel.