Table 1.

Oligonucleotides used in adaptor construction, site-directed mutagenesis, RT-PCR, and SYBR-green quantitative RT-PCR

Oligonucleotides	Nucleotide sequence (5′–3′)	Nucleotide positiona	Polarityb
PBELOASP	TGCACTTAATTAAGCTAGCATGCTAAGCTTCTAGAGGCCTCAG	N/A	+
PBELOASN	AGGCCTCTAGAAGCTTAGCATGCTAGCTTAATTAAG	N/A	−
PBELOXBAP	CCATGCTGGTTCGAGAGAAGGTGTTG	N/A	+
PBELOXBAN	CAACACCTTCTCTCGAACCAGCATGG	N/A	−
BVD424	TGCCGTCACTGCCAGTTA	424–442	+
BVD754	CAAACAAAAACCCGTCGG	736–754	−
BVD6891	AAGACTTGCCGGCCGCTG	6891–6909	+
BVD7920	GCGCTGGTGGCATAGGGG	7902–7920	−
BVD8905	TACTGGGGTCGGGTTCCG	8905–8923	+
BVD9450	GGCCTCGCGTCCTTGTTG	9432–9450	−
BVD9985	GGTGTTACGGTGCCCGCC	9985–10003	+
BVD11155	ACTTGTGTCTGGCTGGCC	11137–11155	−
BVD12043	AAAGGTCCTGCTCATGGC	12043–12061	+
SD1-SdaI-PacI	AGTTAATTAACCTGCAGGGGGCTGTTAGAGG	12294–12308	−

N/A indicates the primers are used for adaptor construction or site-directed mutagenesis.

^aNucleotide positions are in reference to GenBank Accession No. M96751 for BVDV strain SD1.

^b+ and − indicate forward and reverse primers, respectively.