Table 1.

Primers and probes used for detection of conventional enteroviruses as well as the genotypes belonging to the novel HEV-C subspecies

Primer/probe	Sequence	Position (relative to PV1 Mahoney)
HEV F	CCCTGAATGCGGCTAATCC	449–467
HEV R	ATTGTCACCATAAGCAGCCA	593–574
HEV P	FAM-ACGGACACCCAAAGTAGTCGGTTCC-BHQ1	554–530
HEV F_new_C	GCCCCTGAATGTGGATAATCC	447–467
HEV R_new_C	ATTGTCACCATAAACATTCAa	593–574
HEV P_new_C	FAM-CACCCAAAGTAGTTGGTTCCGCCA-BHQ1	558–535

HEV, human enterovirus.

^aZNA oligo (zip nucleic acid). The modification improves affinity for the target sequence and increases the melting temperature of AT-rich oligonucleotides.