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Duplex HRSV rRT-PCR assay limits of detection with RNA transcripts.
| Predicted no. of transcript copies/reaction | No. of positive tests/no. of transcript replicates (%) |
||
|---|---|---|---|
| HRSV A | HRSV B | pan-HRSV | |
| 50 | 16/16 (100) | 16/16 (100) | 16/16 (100) |
| 10 | 16/16 (100) | 16/16 (100) | 16/16 (100) |
| 5 | 14/16 (87.5) | 16/16 (100) | 14/16 (87.5) |
| 2.5 | 11/16 (68.8) | 8/16 (50) | 9/16 (56.3) |
| 1.25 | 6/16 (37.5) | 9/16 (56.3) | 9/16 (56.3) |
