Table 1.

HBoV primers and probe used in viral genome amplification.

Gene	Amplicon	Forward primer	Reverse primer	Size (bp)	Reference
NP1 for real-time PCR	NP-1	5′-AGAGGCTCGGGCTCATATCA-3′	5′-CACTTGGTCTGAGGTCTTCGAA-3′	81	26
	Probe	5′ FAM-AGGACCACCCAATCARCCACCTATCGTCT-BHQ1 3′
NS1 for conventional RT-PCR	NS-1	5′-TATGGCCAAGGCAATCGTCCAAG-3′	5′-GCCGCGTGAACATGAGAAACAG-3′	245	13
VP1 for sequence	VP1	5′-CCACTAGTATGCCTCCAATTAAGAGACAGC-3′	5′-CCTCTAGATTACAACACTTTATTGATGTTTGT-3′	2016
VP2	VP2-P1	5′-CCCAAGCTTGCATGTCTGACACTGACATTCAA-3′	5′-CCGCTCGAGTTAATCACAAAAGATGTGAACGC-3′	480
VP2	VP2-P2	5′-CCCAAGCTTGCATG CAAATACTTTCAAATGGTGCT-3′	5′-CCGCTCGAGTTATGTGCTTCCGTTTTGTCTTAT-3′	435
VP2	VP2-P3	5′-CCCAAGCTTGCATGCTAATGTTTAATCCAAAAGTC-3′	5′-CCGCTCGAGTTA TCTGGTGATAGGAAATCTGTC-3′	465
VP2	VP2-P4	5′-CCCAAGCTTGCATGCTATACAAAGGAAACCAAACC-3′	5′-CCGCTCGAGTTACAACACTTTATTGATGTTTG-3′	474