Table 1.
Kinetic study of coxsackievirus B1-infected PMK cells as demonstrated by flow cytometric analysis, IFA, and observation of CPE. Data for flow cytometry and IFA are from cells stained with coxsackievirus B blend antibody. Flow cytometry detected positively staining, infected cells 1 day prior to IFA and appearance of CPE.
| Flow cytometry—% gated cells staining positively | IFA—semi-quantitative measurementa | CPE—semi-quantitative measurementb | |
|---|---|---|---|
| Day 1 | 0.65 | 0 | 0 |
| Day 2 | 5.2 | 1–2+ | 1–2+ |
| Day 3 | 50.8 | 3+ | 3+ |
| Day 4 | 46.9 | 4+ | 4+ |
a
0 = no staining observed; 1+ = 1–10% of cells show positive staining; 2+ = 11–30% of cells show positive staining; 3+ = 31–70% of cells show positive staining; 4+ = >70% of cells show positive staining.
b
0 = no CPE observed; 1+ = 1–10% of cells show CPE; 2+ = 11–30% of cells show CPE; 3+ = 31–70% of cells show CPE; 4+ = >70% of cells show CPE.