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. 2019 Feb 5;44:44–50. doi: 10.1016/j.mcp.2019.02.002

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Fig. 2 — Standard curves of SYBR Green I -based qPCR generated by plotting the mean Cq values versus 10-fold serial dilutions of the standards over concentrations ranging from 3.46 × 10² to 3.46 × 10⁸ copies/μL for PEDV and 6.12 × 10² to 6.12 × 10⁸ copies/μL for PCV3 in triplicate. The coefficient of determination (R2) and the equation of the regression curve (y) were calculated using a LightCycler^® 96 Instrument (Roche). A and B, single qPCR for PEDV and PCV3; C, duplex qPCR for PEDV and PCV3.