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. 2016 Mar 2;130:19–26. doi: 10.1016/j.antiviral.2016.03.003

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© 2016 Elsevier B.V. All rights reserved.

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Fig. 2 — Growth kinetics and evaluation of the genetic stability of recombinant duck enteritis viruses (rDEVs). (A) Growth kinetics of the rDEVs. Compared with the WT DEV Clone-03, the rDEVs with the US10 deletion exhibited statistically significant lower viral titers (**p < 0.01, *p < 0.05). Viral titers are shown in TCID₅₀/100 μL. (B) The genetic stability of the rDEVs expressing the major structural proteins of infectious bronchitis virus (IBV). The identity of the rDEVs was confirmed by PCR using the differentiated primer pair DF and DR following the 5th, 10th, 15th, and 20th passages. No reversion occurred during the passage from the rDEVs to the WT DEV Clone-03 (DEV Clone-03, 637 bp; rDEV-N, 2217 bp; rDEV-S, 4502 bp; rDEV-S1, 2610 bp).