Table 1.
Primers and probes used for RV-C assays.
| Oligonucleotide | Oligonucleotide sequence (Position) |
|---|---|
| IrlonS (Forward Primer) | 5′-GCACTTCTGTTTCCCC-3′ (165–180)a |
| EntA (Reverse Primer) | 5’-GCATTCAGGGGCCGGAG-3’ (461–445)a |
| RV-TYPE-C Probe 1 | 5′-FAM-CCTGCGTGGCTGCC-MGBNFQ-3′ (358–371) |
| RV-TYPE-C Probe 2 | 5′-FAM-CCCGCGTGGCTGCC3′-BHQ-1 (354–367) |
| RV-TYPE-C Probe 3 | 5′-FAM-CCCGCGTGGTGCCC-MGBNFQ-3′ (377–390) |
| RV-TYPE-C Probe 4 | 5′-FAM-CCTGCGTGGTGCCC3′-BHQ-1 (383–396) |
a
Nucleotide position was determined using this reference sequence [NC_002058.3] from GenBank. LNA bases are underlined.