Table 1.

DENV can replicate independently of a functional 5′ cap under conditions of reduced eIF4E

	Cell treatment
	Untreated	40 μM LY294002	1 μM Wortmannin
m7GpppA-capped DENV RNA (pfu/ml)	822 ± 269	1022 ± 758	2455 ± 1296
ApppA-capped DENV RNA (pfu/ml)	0	183 ± 42	378 ± 270

Equal amounts of functionally (m⁷GpppA) or nonfunctionally (ApppA) capped in vitro transcripts generated from the DENV-2 infectious clone were transfected into cells for 4 h. Cells were washed and incubated with LY294002 or wortmannin for 24 h. Supernatants were then collected and titered on BHK cells.