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Estimation of α-helix and β-sheet relative proportions in SFV21 inserted into a lipid monolayer of DMPC:DMPG:cholesterol:DMPE (6:2:5:3) during compression and decompression (D) (± 5%) obtained from subtracted PMIRRAS spectra.
| Π (mN/m) | Ri = 20 |
Ri = 75 |
||
|---|---|---|---|---|
| 1650 cm− 1 (α-helix) |
1627 cm− 1 (β-sheet) |
1650 cm− 1 (α-helix) |
1627 cm− 1 (β-sheet) |
|
| 5 | 29,5 | 70,5 | 29,5 | 70,5 |
| 15 | 40,5 | 59,5 | 55,5 | 44,5 |
| 25 | 41,0 | 59,0 | – | – |
| 30 | – | – | 62,0 | 38,0 |
| 35 | 48,0 | 52,0 | – | – |
| 15 D | – | – | 74,5 | 25,5 |
| 10 D | 45,0 | 55,0 | – | – |
