Table 2.
Hyper-mutant (HM) VP1 sequences (bases 38-303) derived from transfected HEK293 cells.
| Assay | NGS valid readsa | U-to-C HM |
A-to-G HM |
||||
|---|---|---|---|---|---|---|---|
| Number of reads | Mutations/read (mean, min, max) | Mutation rateb | Number of reads | Mutations/read (mean, min, max) | Mutation rateb | ||
| 1 | 99,982 | 266 | 8.7 (5–28) | 8.7 × 10− 5 | 67 | 7.8 (5–14) | 2.0 × 10− 5 |
| 2 | 99,917 | 481 | 8.0 (5–20) | 1.4 × 10− 4 | 0 | NA | 0 |
| 3 | 99,973 | 697 | 9.2 (5–31) | 2.1 × 10− 4 | 16 | 7.1 (5–18) | 4.3 × 10− 6 |
a
Reads with an average base quality (Sanger-scaled Phred) score Q > 28 at mutated positions.
b
Mutation rates were estimated by dividing, for a giving assay, the total number of mutations by the product of reads times the length of the PCR product (266 bases).