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. 2014 May 4;205:110–115. doi: 10.1016/j.jviromet.2014.04.016

Table 2.

Detection limits of each assay using serial dilutions of in vitro transcribed control viral RNA.

Template RNA concentration (copies/reaction) Number of positive replicates/number of tests for each assay (positive %)
B/NSa B/Vicb B/Yamc
10 6/6 (100) 6/6 (100) 6/6 (100)
5 6/6 (100) 6/6 (100) 6/6 (100)
1 0/6 (0) 1/6 (16.7) 3/6 (50)
0.1 0/6 (0) 0/6 (0) 0/6 (0)

The template RNA for each assay was the NS gene segment of a B/Florida/4/2006 and the HA gene segments of b B/Brisbane/60/2008 and c B/Massachusetts/02/2012.