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. Author manuscript; available in PMC: 2020 Apr 21.
Published in final edited form as: Cell Rep. 2020 Mar 10;30(10):3218–3228.e5. doi: 10.1016/j.celrep.2020.02.053

Figure 4. Myb Is Required for BEAF-32 and CP190 Binding at the Majority of Insulator Sites Tested.

Figure 4.

(A–B′) ChIP-quantitative real-time PCR analysis reveals that the loss of Myb leads to a significant reduction of BEAF-32 binding (A), even though the BEAF-32 protein expression level (A′) is comparable in the control and Myb mutant. Similarly, the loss of Myb leads to a significant reduction of CP190 binding for most insulator sites tested (B) even though CP190 protein expression level (B′) is comparable in the control and Myb mutant. Note that the last three sites depicted in (A) and (B) represent negative controls that overlap BEAF-32 and/or CP190 but not Myb peaks (Nrg, CheB98a, and CG14309-cona for BEAF-32; and Trxt-dhd, Atg4b-CG5044, and CheB98a for CP190). Gene pairs of DPGs are denoted by gene names separated by dashes. Error bars represent mean ± SEM; ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed unpaired Student’s t test. ImageJ software was used to quantify western blot signals.