KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-histone H3K4me3 | Abcam | Cat# ab8580; RRID:AB_306649 |
| Anti-histone H3K27me3 | Abcam | Cat# ab6002; RRID:AB_305237 |
| Anti-histone H3K9me3 | Abcam | Cat# ab8898; RRID:AB_306848 |
| Rabbit Anti-CP190 | Corces laboratory | N/A |
| Rabbit Anti-BEAF-32 | Corces laboratory | N/A |
| Rabbit Anti-Rpb3 subunit of Pol II | Adelman laboratory | N/A |
| Rabbit polyclonal Anti-Myb | Botchan laboratory | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| TRIzol | Invitrogen | Cat#80806 |
| cOmplete, Protease Inhibitor Cocktail | Roche | Cat#19541400 |
| Dynabeads Protein A | Invitrogen | Cat#00324078 |
| RNase A | Invitrogen | Cat#1383185 |
| Proteinase K | Invitrogen | Cat#1250776 |
| Critical Commercial Assays | ||
| RNeasy Mini Kit | QIAGEN | Cat#74104 |
| SuperScript Double-Stranded cDNA Synthesis Kit | Invitrogen | Cat#1225016 |
| PowerUp SYBR Green Master Mix | Applied Biosystems | Ref#100029284 |
| Dual-Color DNA Labeling Kit | NimbleGen | Cat#13678600 |
| SureTag DNA labeling Kit | Agilent | Cat#0006300758 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GSE100143 |
| D. melanogaster reference genome NCBI | Dm3 5.7 | ftp://ftp.flybase.net/genomes/Drosophila_melanogaster/dmel_r5.7_FB2008_04/ |
| Insulator proteins ChIP-chip | modENCODE Consortium et al., 2010 | http://www.modencode.org/ |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: Df(1)MH107, w1118/FM7i, P{w[+mC] = ActGFP}JMR3 | Bloomington Drosophila Stock Center | Cat#30559; RRID:BDSC_30559 |
| D. melanogaster: y1w67c23 | Bloomington Drosophila Stock Center | Cat#6599; RRID:BDSC_6599 |
| D. melanogaster: w[*]; E2f276Q1, cn1, bw1/CyO, P(ry[+t7.2] = ftz/lacB)E3 | Bloomington Drosophila Stock Center | Cat#7436; RRID:BDSC_7436 |
| D. melanogaster: w[*]; Df(2L)G5.1, dpy[ov1] b1/CyO, P(ry[+t7.2] = ftz/lacB)E3 | Bloomington Drosophila Stock Center | Cat#7437; RRID:BDSC_7437 |
| Oligonucleotides | ||
| Primers for quantitative real-time PCR, see Table S5 | This paper | N/A |
| Software and Algorithms | ||
| ArrayStar (Version 12.0.0) | DNASTAR, Inc. | https://www.dnastar.com |
| Agilent Feature Extraction (Version 9.5.1) | Agilent | https://www.agilent.com |
| Agilent Genomics Workbench (Version 7.0) | Agilent | https://www.agilent.com |
| JASPAR | Mathelier et al., 2016 | http://jaspar.genereg.net/ |
| MEME suite | Bailey et al., 2009 | http://meme-suite.org/tools/meme |
| WebLogo | Crooks, et al., 2004 | http://weblogo.berkeley.edu/logo.cgi |
| PRISM Version 8 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| Image Processing and Analysis in Java | ImageJ | https://imagej.nih.gov/ij/index.html |