Table 2.
Potential sites for positive selection of mutations in Spanish strains of porcine reproductive and respiratory syndrome virus GP5
| Position in GP5 | Lelystad virus | Spanish strains 2000–2005 (n = 47)a |
Spanish strains 1991–1995 (n = 17) |
||||
|---|---|---|---|---|---|---|---|
| Aminoacid | Aminoacid (strains) | NS/Sb | Probabilitya | Aminoacid (strains) | NS/Sb | Probabilitya | |
| 10 | Phenilalanine | Serine (14) | 18/3 | 1.3 × 10−20 | No change | 0/0 | N.A. |
| 12 | Threonine | Isoleucine (12) | 20/0 | 1.8 × 10−13 | No change | 0/0 | N.A. |
| 13 | Proline | Glutamine (12) | 21/18 | 1.3 × 10−18 | Leu (1) | 2/8 | 0.03 |
| Leucine (7) | 3.2 × 10−6 | ||||||
| 21 | Phenilalanine | Serine (5) | 6/0 | 7.5 × 10−9 | No change | 0/0 | N.A. |
| 32 | Alanine | Valine (15) | 19/0 | 9.6 × 10−17 | Valine (5) | 5/0 | 3.2 × 10−7 |
| 36 | Glycine | Aspartic acid (12) | 17/2 | 2.1 × 10−17 | Aspartic (2) | 3/0 | 8.0 × 10−3 |
| 37 | Aspartic acid | Asparagine (38) | 0/47 | 1.5 × 10−53 | Asparagine (5) | 9/0 | 1.5 × 10−7 |
| Serine (5) | 9.7 × 10−4 | Serine (4) | 8.9 × 10−6 | ||||
| 46 | Asparagine | Aspartic acid (12) | 15/14 | 1.5 × 10−12 | No change | 0/0 | N.A. |
| 56 | Aspartic acid | Glycine (16) | 42/0 | 6.7 × 10−11 | Glutamic acid (4) | 7/1 | 2.6 × 10−6 |
| Glutamic acid (14) | 4.2 × 10−15 | ||||||
| 60 | Serine | Asparagine (16) | 33/0 | 6.2 × 10−33 | Asparagine (1) | 2/0 | 0.03 |
| 63 | Glycine | Aspartic acid (18) | 45/0 | 1.1 × 10−20 | Aspartic acid (11) | 12/0 | 3.2 × 10−19 |
| Serine (12) | 5.7 × 10−12 | ||||||
| 96 | Glycine | Serine (13) | 15/10 | 7.8 × 10−19 | Serine (2) | 2/1 | 8.0 × 10−4 |
| 100 | Threonine | Isoleucine (11) | 24/0 | 9.1 × 10−11 | Isoleucine (3) | 5/0 | 3.4 × 10−4 |
| 101 | Alanine | Threonine (30) | 46/0 | 1.1 × 10−29 | Threonine (8) | 9/0 | 1.6 × 10−9 |
| 106 | Glycine | Lysine (16) | 26/3 | 4.8 × 10−15 | Lysine (4) | 3/1 | 3.8 × 10−7 |
| 111 | Cysteine | Serine (36) | 47/0 | 1.4 × 10−54 | Serine (15) | 15/0 | 2.1 × 10−27 |
| 116 | Alanine | Valine (17) | 26/1 | 1.5 × 10−18 | Phenilalanine (1) | 1/0 | 0.02 |
| 119 | Phenilalanine | Leucine (9) | 17/10 | 7.0 × 10−12 | Leucine (3) | 3/1 | 1.3 × 10−6 |
| 122 | Phenilalanine | Leucine (30) | 35/0 | 9.3 × 10−42 | Leucine (9) | 9/0 | 9.9 × 10−17 |
| 123 | Valine | Alanine (13) | 19/11 | 8.6 × 10−12 | No change | 0/0 | N.A. |
| 143 | Phenilalanine | Histidine (9) | 12/9 | 4.1 × 10−14 | No change | 0/0 | N.A. |
| 154 | Valine | Isoleucine (39) | 42/0 | 4.9 × 10−66 | Isoleucine (10) | 10/0 | 1.7 × 10−15 |
| 172 | Aspartic acid | Glycine (34) | 41/5 | 2.6 × 10−39 | Glycine (10) | 10/6 | 5.7 × 10−10 |
| 174 | Asparagine | Aspartic acid (35) | 43/0 | 1.7 × 10−52 | Aspartic acid (10) | 11/0 | 1.8 × 10−17 |
a
Probability: probability, according to a binomial distribution, that in a set of n independent sequences with a mutation, a given number of strains shared the same non-synonymous mutation. For strains detected in the period 2000–2005 only mutations with probabilities lower than 1.0 × 10−9 are shown.
b
NS/S: non synonymous/synonymous mutations.