Table 3.

Potential sites for negative selection of mutations in Spanish strains of porcine reproductive and respiratory syndrome virus GP5 obtained in 2000–2005

Position in GP5	Aminoacid	S/NSa	Probabilityb
8	Glycine	20/2	2.0 × 10−24
73	Proline	17/1	1.3 × 10−21
79	Leucine	33/4	1.3 × 10−54
85	Threonine	15/4	9.6 × 10−17
89	Phenilalanine	16/3	3.3 × 10−26
91	Aspartic acid	31/4	3.69 × 10−51
108	Tyrosine	34/8	3.6 × 10−53
109	Valine	21/7	3.9 × 10−22
112	Serine	22/8	3.9 × 10−33
113	Valine	34/8	4.6 × 10−37
135	Alanine	36/5	8.4 × 10−42
137	Arginine	28/5	9.3 × 10−26
140	Arginine	25/7	1.9 × 10−21
153	Arginine	35/5	2.5 × 10−31
160	Proline	16/3	1.2 × 10−18
163	Valine	25/4	5.8 × 10−29
164	Glutamic acid	39/3	4.9 × 10−66
169	Alanine	21/7	3.9 × 10−22
194	Threonine	14/4	1.5 × 10−15
200	Glutamic acid	35/8	7.4 × 10−55

^aS/NS: synonymous/non-synonymous mutations.

^bProbability: probability, according to a binomial distribution, that in a set of n independent sequences with a mutation, a given number of strains shared a synonymous mutation.