Table 4.
Significant changes (p < 0.05) in codon usage for negatively selected sites of ORF5 of porcine reproductive and respiratory strains isolated in 1991–1995 or 2000–2005
| Position in GP5 | Aminoacids | Codons 1991–1995a | Codons 2000–2005a |
|---|---|---|---|
| 73 | Proline | CCG (18/18) | CCG (30/46) |
| CCA (15/46) | |||
| CCC (1/46) | |||
| 79 | Leucine | CTC (12/18) | CTC (11/44) |
| CTT (6/18) | CTT (33/44) | ||
| 85 | Threonine | ACA (18/18) | ACA (29/45) |
| ACG (13/45) | |||
| ACC (3/45) | |||
| 89 | Phenilalanine | TTT (18/18) | TTT (29/46) |
| TTC (17/46) | |||
| 91 | Aspartic acid | GAC (11/18) | GAC (13/45) |
| GAT (7/18) | GAT (32/45) | ||
| 109 | Valine | GTA (15/17) | GTA (20/41) |
| GTG (2/17) | GTG (21/41) | ||
| 112 | Serine | AGC (15/18) | AGC (18/40) |
| AGT (3/18) | AGT (22/40) | ||
| 135 | Alanine | GCC (7/17) | GCC (7/43) |
| GCT (10/17) | GCT (34/43) | ||
| GCG (2/43) | |||
| 140 | Arginine | CGT (13/17) | CGC (16/40) |
| CGC (4/17) | CGC (24/40) | ||
| 153 | Arginine | AGA (8/18) | AGA (8/43) |
| AGG (10/18) | AGG (35/43) | ||
| 160 | Proline | CCA (18/18) | CCA (29/45) |
| CCG (11/45) | |||
| CCC (5/45) | |||
| 163 | Valine | GTA (13/15) | GTA (18/43) |
| GTG (2/15) | GTG (16/43) | ||
| GTC (9/43) | |||
| 164 | Glutamic acid | GAA (16/18) | GAA (6/45) |
| GAG (2/18) | GAG (39/45) | ||
| 169 | Alanine | GCC (10/18) | GCC (20/41) |
| GCT (8/18) | GCT (21/41) | ||
a
Differences in the denominator to 18 (1991–1995 sequences) or to 46 (2000–2005 strains) reflect non-synonymous mutations.