Fig. 4.

Comparison of immunoreactive ficolin subunits of GlcNAc-affinity-purified pFCN and rFCN under 12% SDS-PAGE (reducing) conditions. Preparations of porcine ficolin α purified from plasma (pFCN) contain a major ∼40 kDa band in a 38–42 kDa triplet (A, lane 1) that is immunoreactive with antibodies to ficolin (B, Lane 1) consistent with previous studies (Brooks et al., 2003a, Brooks et al., 2003b). By comparison, GlcNAc-affinity-purified rFCN migrated mainly around 44 kDa (A, lane 2), and was recognized by polyclonal anti-ficolin antibody (B, lane 2) or anti-V5 antibody which detects the C-terminal tag of rFCN (B, lane 4). Plasma-purified ficolin was not detected with anti-V5 antibody (B, lane 3).