Fig. 7.

Comparison of the bacterial-binding activity of GlcNAc-affinity-purified pFCN and rFCN in suspension cultures of intact bacteria (Actinobacillus pleuropneumoniae serotype 5b and Salmonella enteritidis) that are known to bind porcine ficolin α. Bound proteins were eluted with GlcNAc (300 mM) after prior washing to remove non-specifically bound proteins, and eluted proteins were separated by12% SDS-PAGE (reducing) and detected by immunoblots with polyclonal anti-ficolin antibody. Lanes 1, 4, 7 and 10 are unbound fractions, lanes 2, 5, 8 and 11 are the final wash fractions, and lanes 3, 6, 9 and 12 are the GlcNAc-eluted proteins.