Fig. 8.

Inhibition of PRRSV replicative efficiency by pFCN or rFCN in plaque assays. Panel A shows plaques in monolayer cultures of Marc-145 cells in 60 mm dishes fixed and stained with crystal violet. Panel B shows numbers of plaques per dish expressed as a percent (±S.D.) of numbers of plaques in cultures with PRRSV alone. Cultures were incubated for 72 h after infection with PRRSV alone, or with PRRSV pre-incubated with either 100 mM GlcNAc or anti-ficolin antibody. The number of visible plaques were significantly reduced in the presence of either 10 μg/ml of pFCN (p = 0. 002) or rFCN (p = 0.009), and their effects were similarly blocked by pre-incubation of PRRSV with either 100 mM GlcNac or anti-ficolin antibody. Each bar is the mean (±S.D.) from three separate experiments, each culture in duplicate.